邹沫君1，陈诚2

（1 乐山市食品药品检验检测中心，四川乐山614000；2山东高速四川产业运营公司，四川乐山614000）

摘要：建立了高效液相色谱-蒸发光散射法测定饮料中果糖、葡萄糖、麦芽糖及乳糖含量的方法。用水作为提取剂，以乙腈-水（体积比70∶30）为流动相，采用Ultimate® XB-NH2柱（250mm×4.6mm，5μm）进行分离，蒸发光散射检测器检测。结果表明：4种糖在2.0～10.0mg/mL均有良好的线性关系，相关系数（r）为0.9999；方法检出限为0.33g/kg～0.82g/kg；3 个不同水平（2.0、4.0、8.0g/kg）的加标平均回收率为96.00%～102.50%，RSD值为1.14%～2.04%。该方法前处理简单、灵敏度高、重现性佳、检测速度快，适用于饮料中4 种糖的同时检测。

关键词：高效液相色谱；饮料；果糖；葡萄糖；麦芽糖；乳糖

Determination of Fructose，Glucose，Maltose and Lactose in Beverages by High PerformanceLiquid Chromatography

ZOU Mo-jun1，CHEN Cheng2

（1 Leshan Center for Food and Drug Control，Leshan 614000，China；2 Shandong Highspeed Sichuan Industrial Operation Company，Leshan 614000，China）

Abstract: To establish a method for determination of fructose，glucose，maltose and lactose in beverages by highperformance liquid chromatography- evaporative light scattering（HPLC- ELSD）. The samples were extracted withwater. The separation of targeted compounds was performed on a Ultimate® XB-NH2 column（250mm×4.6mm，5μm）using acetonitrile-water（70∶30，v/v）as mobile phase，detected by evaporation light scattering detector（ELSD）. Theresults showed that the 4 kinds of sugars had good linearity in the concentration range of 2.0～10.0mg/mL，and thecorrelation coefficients were 0.9999；the limit of detection was 0.33g/kg～0.82g/kg；and the standard recovery rate（standard additive concentration is 2.0，4.0，8.0g/kg） was between 96.00% ～102.50% ，and relative standarddeviation（RSD）was from 1.14% to 2.04%. This method has the advantages of simple pretreatment，high sensitivity，good reproducibility and fast detection speed，which is suitable for quantitative detection of fructose，glucose，maltose and lactose in beverages.

Key words: high performance liquid chromatography；beverages；fructose；glucose；maltose；lactose

中图分类号：TS207.3 文献标志码：A 文章编号：1007-7871（2020）05-0066-04

（2020年 第23卷 第5期）