李亚，梁剑锋*，梁峰

（梧州市食品药品检验所，广西梧州543002）

摘要：该文采用高效液相色谱法测定饮料中的人工合成着色剂柠檬黄，对国家标准GB 5009.35-2016 中的聚酰胺吸附法进行方法改进后，在以下色谱条件下测定：采用ACE Excel 2 AQ型（50×2.1mm）色谱柱；流动相为97%0.02mol/L乙酸铵：3%甲醇；流速为0.35mL/min。结果：柠檬黄在0.50～5.00μg/mL范围内呈良好线性关系，检出限和定量限分别为0.49mg/kg 和1.47mg/kg，在7.5μg~75μg 加标范围时回收率为89.69%～102.56%，该实验研究方法的相对标准偏差（RSD）均小于2%。

关键词：果汁饮料；柠檬黄；高效液相色谱

Study on the Detection Method of Lemon Yellowin Fruit Juice Drinks

LI Ya，LIANG Jian-feng*，LIANG Feng

（Wuzhou Food and Drug Inspection Institute，Wuzhou 543002，China）

Abstract: In this paper，the synthetic colorant lemon yellow in beverages was determined by high performance liquidchromatography. After improving the method of polyamide adsorption in national standard GB 5009.35 -2016，thefollowing chromatographic conditions were adopted: ACE Excel 2 AQ (50×2.1mm) column，mobile phase 97%0.02mol/Lammonium acetate: 3%methanol. The speed is 0.35mL/min. Result：Lemon yellow showed a good linear relationship in therange of 0.50~5.00μg/mL.The detectionlimit and quantitative limitwere 0.49mg/kgand 1.47mg/kg，respectively. The recoveryrate was 89.69%~102.56%in the range of 7.5μg~75.0μg. The relative standard deviation（RSD）of the experimental methodwas less than 2%.

Key words: fruit juice beverage；tartrazine；high performance liquid chromatography

（2018年第21卷第6期）