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一种测定果汁饮料中7 种合成色素的HPLC方法

邹沫君1,陈诚2
(1 乐山市食品药品检验检测中心,四川乐山614000;
2山东高速四川产业运营公司,四川乐山614000)

摘要:建立了同时测定果汁饮料中7 种合成着色剂(柠檬黄、新红、苋菜红、胭脂红、日落黄、亮蓝、赤藓红)的固相萃取柱净化高效液相色谱方法。试样经无水乙醇-氨水溶液-水(体积比7∶2∶1)提取,Cleanert PWAX固相萃取SPE柱富集和净化后,采用Nvcleodur C18柱(250mm×4.6mm,5μm),以0.02mol/L 乙酸铵-甲醇为流动相进行梯度洗脱,采用二极管阵列检测器,多波长检测定量。结果表明:7 种合成着色剂在0.5~40.0μg/mL均有良好的线性关系,相关系数(r)为0.9999;方法检出限为0.025~0.097mg/kg;3 个不同水平(2.0、5.0、10.0mg/kg)的加标平均回收率为96.51%~
104.41%,RSD值为0.95%~2.01%。该方法具有操作简便、灵敏度高、重现性佳等特色,适用于果汁饮料中7 种合成着色剂的同时检测。

关键词:高效液相色谱;果汁饮料;合成着色剂;固相萃取

 


Simultaneous Determination of 7 Synthetic Colorants in Fruit Juice Drinks by High Performance Liquid Chromatography

ZOU Mo-jun1,CHEN Cheng2
(1 Leshan Center for Food and Drug Control,Leshan 614000,China;
2 Shandong Highspeed Sichuan Industrial Operation Company,Leshan 614000,China)

Abstract: A method was developed for simultaneous determination of 7 synthetic colorants(lemon yellow,new red,amaranth,carmine,sunset yellow,brilliant blue,erythrosine)in fruit juice drinks by PWAX solid phase extraction -HPLC. In the experiments,the samples were extracted by ethanol-ammonia-water(7∶2∶1 v/v),then the extract was purified and concentrated by Cleanert PWAX(SPE) column,and 7 synthetic colorants were separated by Nvcleodur C18 Column(250mm × 4.6mm,5μm) with 0.02mol/L ammonium acetate - methanol as mobile phase in gradient elution, detected by 200~800nm multi- wavelength PDA method. The results shows that 7 synthetic colorants had good liner relations when the concentration was in the range of 0.5~40.0μg/mL and the correlation coefficients were 0.9999. The detection limit was from 0.025 to 0.097mg/kg. The recovery of the method(standard additive concentration is 2.0,5.0,10.0mg/kg)was from 96.51% to 104.41%,and the relative standard deviation (RSD) was from 0.95% to 2.01% . The method is simple and convenient,good reproducibility,suitable for 7 synthetic colorants simultaneous determination in fruit juice drinks.

Key words: high performance liquid chromatography;fruit juice drinks;synthetic colorant;solid phase extraction

(2020年 第23卷 第3期)

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