曾 慧，张明珠

（清远加多宝草本植物科技有限公司，广东 清远 523000）

摘要：目的：采用液液萃取+固相萃取净化的前处理方法，运用高效液相色谱法测定菊花和金银花中吡虫啉的残留量。方法：样品经甲醇超声提取后，液液萃取+固相萃取净化，使用紫外可变波长检测器和Waters Atlantis T3 柱，以甲醇和水为流动相，在268nm 波长下检测样品中吡虫啉残留量。结果：在0.10mg/L～2.00mg/L 浓度范围内，曲线相关系数为0.9999，回收率为85%～95%，RSD 为0.48%～2.68%，方法检出限为0.10mg/kg。结论：方法具有净化效果好、精密度和准确度高的特点。

关键词：吡虫啉；液液萃取；固相萃取；高效液相色谱

Determination of Imidacloprid Residues in Chrysanthemum and Honeysuckle by HPLC

ZENG Hui，ZHANG Ming-zhu

（Qingyuan jiadobao herbal Technology Co.，Ltd.，Qingyuan 523000，China）

Abstract: Objective：To determine the residues of imidacloprid in chrysanthemum and Honeysuckle by high performanceliquid chromatography（HPLC）with liquid-liquid extraction（LME）and solid phase extraction（SPE）. Methods:Afer ultrasonic extraction with methanol，the samples were purified by liquid-liquid extraction（lme）and HLBsolid phase extraction（spe）. The residue of imidacloprid in the samples was detected at 268nm using methanol andwater as mobile phase using a uv variable wavelength detector and an Atlantis T3 column. Results：In the concentrationrange of 0.20mg/L～2.00mg/L，the curve correlation coefficient was 0.9999，the recovery rate was 85%～95%，RSD was 0.48%～2.88%，and the detection limit was 0.10mg/kg. Conclusion：The method had the characteristics ofgood purification effect，high precision and accuracy.

Key words: imidacloprid；liquid-liquid extraction；solid phase extraction；high performance liquid chromatography；carbendazims

中图分类号：O657.7+2             文献标志码：A                   文章编号：1007-7871（2023）01-0022-03

2023，Vol.26，No.1