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液液萃取-高效液相色谱法测定红茶中赭曲霉毒素A

曾 慧,张明珠

(清远加多宝草本植物科技有限公司,广东 清远 523000)

摘要:目的:建立一种液液萃取净化的高效液相色谱法测红茶中的赭曲霉毒素A 的方法。方法:试样经60%乙腈超声提取,液液萃取净化,以甲醇~0.5%乙酸梯度洗脱,C18 短柱分离后经高效液相色谱仪-荧光检测器检测。结果:赭曲霉毒素A 在10.00μg/L~50.00μg/L 浓度范围,相关系数为1.00000,加标回收率为83%~98%,RSD 为1.11%~2.47%,检出限为0.0052mg/kg。结论:仪器20min 完成一个样品测定,方法检测速度快,同时具有净化效果好,精密度和准确度高的特点。

关键词:红茶;赭曲霉毒素A;高效液相色谱;液液萃取

Determination of Ochratoxin A in Black Tea by Liquid-Liquid Extraction HPLC

ZENG Hui,ZHANG Ming-zhu(Qingyuan Jiaduobao Herbal Technology Co.,Ltd.,Qingyuan 523000,China)

Abstract: Objective: A method for determination of ochratoxin A in black tea by liquid extraction and purificationwith high performance liquid chromatography was established. Methods:The samples were ultrasonically extractedby 60% acetonitrile,purified by liquid-liquid extraction,eluted by gradient elution of methanol~0.5% acetic acid,separated by C18 short column and detected by high performance liquid chromatography-fluorescence detector. Results:The concentration of ochratoxin A ranged from 10.00μg/L to 50.00μg/L;the correlation coefficient was 1.00000,therecoveries were 83% to 98%;RSDS were 1.11% to 2.47%;and the detection limit was 0.0052mg/kg. Conclusion:The method can complete the determination of a sample in 20 minutes,which has the characteristics of fast detectionspeed,good purification effect,high precision and accuracy.

Key words: black tea;ochratoxin A;high performance liquid chromatography;liquid-liquid extraction

中图分类号:O657.7+2                  文献标志码:A                      文章编号:1007-7871(2023)02-0025-04

2023,Vol.26,No.2

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