杨帛，刘锐萍，刘浩，张克义，赵广西

（国家葡萄、葡萄酒质量监督检验中心（秦皇岛） /秦皇岛市食品质量安全监督检验中心，河北秦皇岛  066000）

摘要：同时检测食品中的对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、苯甲酸、山梨酸、脱氢乙酸等6 种防腐剂，对色谱条件及样品的前处理方法进行研究，实现了前处理条件及色谱条件的一致性，利用高效液相色谱仪的梯度洗脱和波长切换，实现了6 种防腐剂的有效分离。方法回收率高，精密度和检出限均可满足食品安全检测工作需要。是一种简便、快捷、准确的检测方法。

关键词：多种防腐剂；高效液相色谱法；同时检测；优化研究

Methods Optimization Research of Simultaneous Determination of Preservatives in Food

YANG Bo，LIU Rui-ping，LIU Hao，ZHANG Ke-yi，ZHAO Guang-xi

（National Center of Supervision&Inspection on Grape andWine Quality/Qinhuangdao Supervision ＆ Inspection instituteon Product Quality，Heibei Qinhuangdao 06600）

Abstract: To simultaneous determinate methylparaben，ethylparaben，eropylparaben，benzoic acid，sorbic acid，anddehydroacetic acidin foods，the chromatographic condition and pretreatment methods of the samples were studied toachieve consistency of the pretreatment conditions and the chromatographic condition of different food preservatives，and gradient elution and wavelength switching in HPLC were conducted to effectively separate 6 food preservatives infoods. The recovery rate of this method is high and the precision and detection limit meet the food safety testing needs.The method is very simple，quick and accurate.

Key words: preservatives；HPLC method；simultaneous determination；optimization research

（2014年第17卷第11期）