曾慧，麦敏华，林杰，王念彬，张明珠

（清远加多宝草本植物科技有限公司，广东 清远 523000）

摘要：目的：对高效液相色谱法测定金银花中吡虫啉残留量的不确定度进行评定。方法：建立高效液相色谱法测定金银花中吡虫啉残留量的不确定度模型，分析影响测量不确定度的主要因素，包括标准储备液的配制、系列标准工作溶液的配制、标准曲线拟合、样品处理、测量重复性，计算合成标准不确定度和扩展不确定度。结果：该方法测量不确定度主要来源于校准曲线的配制，其次为系列标准曲线的拟合。结论：当样品中吡虫啉残留量为0.91mg/kg时，扩展不确定度为0.04mg/kg（k=2，包含因子P=95%）。

关键词：不确定度评定；金银花；高效液相色谱；吡虫啉

Evaluation of Uncertainty in the Determination of Residues in Honeysuckle Lonicera Japonica by HPLC

ZENG Hui，MAI Min-hua，LIN Jie，WANG Nian-bin，ZHANG Ming-zhu

（Qingyuan Jiaduobao Herbal Technology Co.，Ltd.，Qingyuan 523000，China）

Abstract: Objective: To evaluate the uncertainty in the measurement of imidacloprid residues in honeysuckle by highperformanceliquid chromatography. Method: Establish an uncertainty model for the determination of imidaclopridresidues in Lonicera japonica by high-performance liquid chromatography，analyze the main factors affecting measurementuncertainty，including the preparation of the standard stock solution，series of standard working solutions，standard curve fitting，sample processing，measurement repeatability，calculate the synthetic standard uncertainty andexpanded uncertainty. Result: The measurement uncertainty of this method mainly comes from the preparation ofcalibration curves，followed by the fitting of series standard curves. Conclusion: When the residual amount ofimidacloprid in the sample is 0.91 mg/kg，the expanded uncertainty is 0.04 mg/kg（k=2，coverage factor P=95%）.

Key words: evaluation of uncertainty；honeysuckle；high performance liquid chromatography；imidacloprid

中图分类号：O657.7+2        文献标志码：A       文章编号：1007-7871（2023）03-0025-05

2023，Vol.26，No.3