侯兆乾1，王彦平1*，巴根纳1，韩之皓1，杨瑞冬1，金磊1，张祁1，2，张红雨1，2
（1 内蒙古伊利实业集团股份有限公司，内蒙古呼和浩特 010000；
2 内蒙古乳业技术研究院，内蒙古呼和浩特 010000）
摘要：为了研究燕麦谷物饮品蒸汽浸入式杀菌工艺对微生物杀菌的有效性，本研究从未脱壳的燕麦中筛选出耐热菌种，并选定检出频次较高的枯草芽孢杆菌（Bacillus Subtilis）作为目标菌种，采用毛细管法检测了孢子状态下枯草芽孢杆菌在燕麦谷物饮品介质中的D 值、Z 值，将孢子悬液反接到燕麦谷物饮品介质中使菌浓度达到106CFU/mL，然后进行杀菌挑战实验，后续使用无菌培养基进行设备清洗、消毒的有效性验证。结果表明，枯草芽孢杆菌芽孢悬液在燕麦谷物饮品介质中D95℃=5.48min、D100℃=1.67min、D103℃=0.80min、D105℃=0.37min、D110℃=0.10min，Z 值=8.54℃；采用145℃/3s 的杀菌参数可以有效杀灭枯草芽孢杆菌，设备CIP 清洗和121℃/40min 的消毒操作可有效杀灭系统中残留的微生物。
关键词：植物饮品；挑战实验；食品微生物；杀菌工艺；枯草芽孢杆菌
Application and Practice of Challenge Experiment in Sterilization Parameter Designing of Oat Beverage
HOU Zhao-qian1，WANG Yan-ping1*，BA Gen-na1，HAN Zhi-hao1，YANG Rui-dong1，JIN Lei1，ZHANG Qi1，2，ZHANG Hong-yu1，2
（1 Inner Mongolia Yili Industrial Group Co.，Ltd.，Hohhot 010110，China；
2 Inner Mongolia Dairy Technology Research Institute Co.，Ltd.，Hohhot 010110，China）
Abstract: In order to study the effectiveness of steam infusion sterilization process for oat grain beverage onmicrobial disinfection，this study selected heat-resistant strains from unhulled oats，and the Bacillus subtilis with highdetection frequency was selected as the target strain. The D-value and Z-value of Bacillus subtilis spores in oat grainbeverage medium were detected using the capillary method. The bactericidal challenge test was performed with theoat cereal beverage which the bacteria concentration reached 106CFU/mL by add the spores suspension in it. And theeffectiveness of equipment cleaning and disinfection was verified with sterile medium after the sterilization challengetest. The results showed that the D-values of Bacillus subtilis spore suspension in oat grain beverage medium wereD95℃=5.48min，D100℃=1.67min，D103℃=0.80min，D105℃=0.37min，and D110℃=0.10min，and the Z-value was 8.54℃.TheBacillus subtilis was eliminated effectively with sterilization treatment by 145℃/3s. Also the CIP procedure anddisinfection operation by 121℃/40min could kill the residual microorganisms in the system.
Key words: plant-based beverage；challenge test；food microorganisms；sterilization process；bacillus subtilis
中图分类号：TS201.1 文献标志码：A 文章编号：1007-7871（2023）04-0005-07
2023，Vol.26，No.4