徐冬梅1，郭占魁2，李金娟1*
（1 山东鼎科检测技术有限公司，山东潍坊262200；
2 山东龙港硅业科技有限公司，山东 潍坊 261300）
摘要：建立同时测定饮料中6 种有机酸的高效液相色谱法。采用Waters RP18 色谱柱（4.6mm×250mm，5μm），流动相以乙腈：0.1%磷酸溶液（pH 2.24）=5∶95（V/V）等度模式程序，流速0.5mL/min，紫外检测器波长为210nm，柱温30℃。结果表明，优化条件后乳酸、酒石酸、富马酸、柠檬酸、丁二酸、苹果酸6 种有机酸能有效分离，相关系数r分别为1.0000，1.0000，1.0000，0.99996，0.99998，0.99996。平均回收率分别为97.54%，97.51%，97.11%，97.35%，101.1%，97.8%，相对标准偏差（RSD）均<3.0%。该方法灵敏度高、准确性好。本研究为食品质量的综合控制提供了有益的信息。
关键词：饮料；高效液相色谱；有机酸；检测
Determination of 6 Kinds of Organic Acids in Drinks by HPLC
XU Dong-mei1，GUO Zhan-kui2 ，LI Jin-juan1*
（1 Shandong Dingke Testing Technology Co.，Ltd.，Weifang 262200，China；
2 Shandong Longgang Silicon Technology Co. ，Ltd.，Weifang 261300，China）
Abstract: The aim was to develop a HPLC method for simultaneous determination of six organic acids in drinks. TheWaters RP18 column （4.6mm× 250mm，5microm）was adopted with acetonitrile and 0.1% phosphoric acid as themobile phase at a equal mode program（pH 2.24）. The flow rate was 0.5mL/min，The wavelength of the ultravioletdetector is 210nm，and column temperature was 30℃.The results showed that after optimizing the conditions，thetartaric acid，lactic acid，citric acid，succinic acid，and fumaric acid can be effectively separated，correlationcoefficient were 1.0000，1.0000，1.0000，0.99996，0.99998，0.99996 respectively. The average recoveries the correlationcoefficients were 97.54%，97.51%，97.11%，97.35%，101.1%，97.8%，respectively. The relative standard deviations（RSD） were all less than 3.0% . The method has high sensitivity and accuracy. This work provided helpfulinformation for comprehensive quality control of food.
Key words: drink；HPLC；organic acid；determination
中图分类号：O657.7 文献标志码：A 文章编号：1007-7871（2023）05-0036-05
2023，Vol.26，No.5