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离子色谱测定无糖可乐中的甜蜜素

余家胜1,李 瑞1,杨佳雯1,郭 伟1,吴伟都1,2,吴 琴1,2*

(1 杭州娃哈哈集团有限公司,浙江 杭州 310018;

2 浙江省食品生物工程重点实验室,浙江 杭州 310018)

摘要:本文对无糖可乐中的甜蜜素含量测定方法进行研究,优化了色谱分离条件,建立了离子交换-电导法测定无糖可乐中的甜蜜素的方法。该方法使用的色谱柱是Thermo IonPac AG11-HC(50mm×4mm)保护柱以及ThermoIonPac AS11-HC(250mm×4mm)分离柱,KOH 梯度淋洗,抑制电导检测。结果表明,甜蜜素在0.112mg/L~112mg/L时有良好的线性关系,相关系数在0.999 以上;方法检出限为0.05mg/L,系统相对偏差在2%以内,回收率98.22%~103.9%。该方法操作简便,分析快速,重现性好,结果准确、可靠,适用于无糖可乐中甜蜜素的含量测定。

关键词:离子色谱;电导;无糖可乐;甜蜜素

Determination of Sodium Cyclamate in Diet Coke by Ion Chromatography

YU Jia-sheng1,LI Rui1,YANG Jia-wen1,GUO Wei1,WU Wei-du1,2,WU Qin1,2*

(1 Hangzhou Wahaha Group Co,Ltd,Hangzhou 310018,China;

2 Key Laboratory of Food and Biological Engineering of Zhejiang Province,Hangzhou 310018,China)

Abstract: The study reported the establishment of an ion chromatography method to determine sodium cyclamate indiet coke by ion chromatography using conductivity detector. The separation was achieved on a Thermo IonPac AG11-HC guard column and a Thermo IonPac AS11-HC analytical column with KOH as the gradient eluent and suppressedconductivity detector. Results showed that sodium cyclamate had good liner relations when the concentration was inthe range of 0.112mg/L~112mg/L and the correlation coefficient was above 0.999. The detection limit was 0.05mg/L.The system relative deviation was less than 2%,and the recovery for diet coke was in the range of 98.22%~103.9%.The method is simple,rapid,sensitive,accurate and reproducible. It was suitable for determination of sodiumcyclamate in diet coke.

Key words: ion chromatography;conductivity;diet coke;sodium cyclamate

中图分类号:TS27      文献标志码:A        文章编号:1007-7871(2024)01-0022-05

2024,Vol.27,No.1

 

 

 

 

 

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