凌 玲，周 雨，袁 媛，安 娜

（北京市石景山区疾病预防控制中心理化科，北京 100043）

摘要：目的建立使用高效液相色谱仪同时检测特殊用途饮料中的咖啡因、糖精钠、安赛蜜、柠檬黄、胭脂红、日落黄等6 种食品添加剂的方法。方法仪器使用C18 色谱柱，流动相采用甲醇与0.02mol/L 乙酸铵溶液（不需要调节pH 值），经梯度洗脱，通过四个检测波长通道同时采集色谱图和光谱图进行检测分析。结果上述6 种食品添加剂线性范围在1.00 mg/L ～100.0mg/L，相关系数均在0.9999 以上，回收率为91.0%～108.2%。结论本方法操作简单、快速，可用于同时检测特殊用途饮料中的咖啡因等6 种食品添加剂。

关键词：高效液相色谱法；特殊用途饮料；咖啡因；合成着色剂；同时检测

Simultaneous Detection of Six Common Food Additives（Caffeine tec.）in Special Purpose BeveragesUsing High-performance Liquid Chromatography

LING Ling，ZHOU Yu，YUAN Yuan，AN Na

（Shijingshan District Center for Disease Control and Provention，Beijing 10043，China）

Abstract: Objective To establish a method using high- performance liquid chromatography（HPLC） for thesimultaneous detection of six food additives，namely caffeine，sodium saccharin，aspartame，tartrazine，erythrosine，and sunset yellow，in functional beverages. Methods The instrument employed a C18 chromatographic column and amobile phase composed of methanol and a 0.02mol/L solution of ammonium acetate （pH not adjusted）. Thecompounds were eluted by gradient elution，and chromatograms and spectra were simultaneously collected using fourdetection wavelength channels. Results The linear range for the six food additives was 1.00 to 100.0mg/L，withcorrelation coefficients all above 0.9999，and recovery ratios ranging from 91.0% to 108.2% . Conclusion Thismethod is simple，rapid，and suitable for the simultaneous detection of caffeine and the other five other foodadditives in functional beverages.

Key words: high-performance liquid chromatography；functional beverages；caffeine；synthetic colorants；simultaneousdetection

中图分类号：TS207.3                           文献标志码：A                            文章编号：1007-7871（2024）03-0030-05

2024，Vol.27，No.3