郭金颖，赵冬梅*

（秦皇岛市食品药品检验中心，河北 秦皇岛 066000）

摘要：建立了黑果腺肋花楸酒中6 种酚类物质的高效液相色谱-质谱测定方法，样品经10%乙醇稀释后使用高效液相色谱-串联质谱仪进行检测，在正离子多反应监测模式下采集，外标法定量。结果表明，绿原酸、表儿茶素、槲皮素、芦丁、白藜芦醇、矢车菊素6 种酚类物质在10.00-500.00ng/mL 浓度范围内线性关系良好，相关系数R2 均大于0.999，定量限均为10ng/mL；每种物质的低、中、高3 个浓度水平的加标回收率在84.7%～98.0%之间，相对标准偏差在1.1%～6.7%之间。该方法前处理简便、检测结果准确、可靠，可用于花楸酒中酚类物质的同时检测。

关键词：黑果腺肋花楸酒；酚类物质；高效液相色谱-质谱

Determination of Phenolic Substances in Aronia Melanocarpa Wine by HPLC - Tandem Mass Spectrometry

GUO Jin-ying，ZHAO Dong-mei*

（Qinhuangdao Food and Drug Inspection Center，Qinhuangdao 066000，China）

Abstract: In this study，a HPLC-mass spectrometry method was established for the detection of 6 bioactive phenolicsubstances in Aronia melanocarpa wine. The method conditions were：as follows: ESI source positive ion mode，ionspray voltage 5000V，ion source temperature 550℃，gas curtain gas pressure 45 psi，and detection time 10 min.Results: The R2 of the curves of 6 phenolic substances was greater than 0.999. The recoveries of three levels of eachsubstance ranged from 84.7% to 98.0%，the relative standard deviations ranged from 1.1% to 6.7%，and the limits ofquantification were 10ng/mL. Conclusion: The method is simple， accurate and reliable， and can be used forsimultaneous detection of bioactive phenols in Aronia melanocarpa wine.

Key words: aronia melanocarpa wine；bioactive phenols；performanceliquid chromatography-tandem mass spectrometry

中图分类号：TS207                  文献标志码：A                              文章编号：1007-7871（2024）04-0024-05

2024，Vol.27，No.4