辛 刚，党敏花

（国投中鲁果汁股份有限公司，北京 100037）

摘要：建立了同时检测果汁中的乳酸、富马酸和羟甲基糠醛的高效液相色谱分离方法。采用YMC-pack ODSAQ色谱柱（4.6mm×250mm，5μm），流动相为0.03%磷酸、2%甲醇水溶液，流速为0.4 mL/min～0.8mL/min，柱温为35℃。实验结果表明，乳酸和富马酸在210nm 处、羟甲基糠醛在280nm 处得到有效分离，相关系数分别为0.99951、0.99939 和0.99999。回收率分别为95.97％、98.54%和96.50％。该方法被成功地用于同时定量测定浓缩苹果汁中的乳酸、富马酸和羟甲基糠醛。

关键词：液相色谱法；乳酸；富马酸；羟甲基糠醛

Simultaneous Determination of Lactic Acid，Fumaric Acid and Hydroxymethyl Furfural in ConcentratedApple Juice by Liquid Chromatography

XIN Gang，DANG Min-hua

（SDIC Zhonglu Fruit Juice Co.，Ltd.，Beijing 100037，China）

Abstract: A HPLC method for simultaneous determination of lactic acid，fumaric acid and hydroxymethyl furfural infruit juice was developed. The chromatography was performed on an YMC-pack ODS-AQ column（4.6mm×250mm，5μm）with mobile phase consisting of 0.03% phosphoric acid and 2% methanol solution. The flow rate was 0.4mL/min ~ 0.8mL/min and the column temperature was 35℃. The results showed that lactic acid and fumaric acid wereeffectively separated at 210nm and hydroxymethyl furfural at 280nm，and the correlation coefficients were 0.99951、0.99939 and 0.99999，respectively. The recoveries were 95.97％、98.54% and 96.50％，respectively. The method hasbeen successfully used for simultaneous quantitative determination of lactic acid，fumaric acid and hydroxymethylfurfural in concentrated apple juice.

Key words: liquid chromatography；lactic acid；fumaric acid；hydroxymethyl furfural

中图分类号：TS275.5                    文献标志码：A                           文章编号：1007-7871（2024）04-0014-05

2024，Vol.27，No.4