王镓萍1，王雅琼1，程 辉2，吴 琴1，罗 洁1，吕桂善1，张奕敏1*
（1 杭州娃哈哈集团有限公司，杭州娃哈哈科技有限公司，浙江省食品生物工程重点实验室，浙江 杭州 310018；
2 娇时日化（杭州）股份有限公司，浙江 杭州 311222）
摘要：为探究异味奶粉是否存在耐热芽孢杆菌污染，本实验通过对异味奶粉进行100℃，10min 热处理，并结合微生物分离纯化和形态观察共筛选得到6 株耐热芽孢杆菌，经生化鉴定均为热葡糖苷酶地芽孢杆菌，并对其进行耐热性和耐酸性研究。结果表明：经118℃/10min 热处理后除分离株HP3 仍有芽孢残留，其余菌株均被100%杀灭；各分离株在pH ≥ 4.5 时均可良好生长，而在pH≤3.5 时大部分菌株的生长都受到抑制，仅分离株HP3 在pH 为2.5 时仍可缓慢生长。以耐受性最强的分离株HP3 为实验对象，采用汽化过氧化氢消毒装置可完全杀灭空间内不同点位的钢片上接种的热葡糖苷酶地芽孢杆菌，杀菌效率高达6lg。实验为有效防控奶粉生产车间中热葡糖苷酶地芽孢杆菌的污染提供参考依据。
关键词：异味奶粉；热葡糖苷酶地芽孢杆菌；耐热性；耐酸性；汽化过氧化氢
Isolation，Identification and Environmental Tolerance of Geobacillus thermoglucosidasius fromOdorous Milk Powder
WANG Jia-ping1，WANG Ya-qiong1，CHENG Hui2，WU Qin1，LUO Jie1，LV Gui-shan1，ZHANG Yi-min1*
（1 Hangzhou Wahaha Group Co.，Ltd，Hangzhou Wahaha Technology Co.，Ltd，Key Laboratory of Food andBiological Engineering of Zhejiang Province，Hangzhou 310018，China；
2 Jotim Daily Chemical（Hangzhou）Co.，Ltd，Hangzhou 311222，China）
Abstract: In order to investigate whether the odorous milk powder was contaminated by thermophilic bacillus，it wasfirst heated in boiling water at 100℃ for 10min，and then six heat- resistant isolates were acquired by isolation，purification and morphological observation. All isolates were identified as Geobacillus thermoglucosidasius，and theirheat and acid tolerance were studied. The results exhibited that all spores of isolates except HP3 were 100% killed byheat treatment at 118℃ for 10min. All isolates could grow well at pH≥4.5，while most strains were inhibited at pH≤3.5， and only HP3 could grow slowly at pH2.5. The vaporized hydrogen peroxide disinfection device couldcompletely kill the isolate HP3 with better environmental tolerance inoculated on steel plates at different positions inthe space，with a sterilization efficiency of up to 6lg. The results offered a reference basis for effectively preventingand controlling the contamination of G. thermoglucosidasius in the workshop for milk powder production.
Key words: odorous milk powder；Geobacillus thermoglucosidasius；heat tolerance；acid tolerance；vaporizedhydrogen peroxide
中图分类号：TS252.7 文献标志码：A 文章编号：1007-7871（2024）05-0014-05
2024，Vol.27，No.5