杨 静，刘春丽*，黎俊威，胡 杰，廖宇琼

（捷通（广州）检测技术服务有限公司，广东 广州 510730）

摘要：目的：建立反向液相色谱法测定食品中透明质酸钠的分析方法。方法：含透明质酸钠的样品，根据基质的不同，可采用直接提取法或醇沉提取法，提取完的样品溶液经过与透明质酸酶反应，再用高效液相色谱仪分析。色谱柱为MCI GEL CK08EH（8mm×300mm，9μm），流动相：1%磷酸溶液，流速为0.6mL/min，柱温为40℃，进样体积20μL，检测波长232nm，外标法定量。结果：透明质酸钠的相关系数为1.0000，加标回收率为93.2％～100.3%，相对偏差为2.71%和3.26%，检出限为0.3mg/kg，定量限为1.0mg/kg。结论：该方法适用范围广，准确度、精密度良好，适用于食品中透明质酸钠含量的测定。

关键词：高效液相色谱法（HPLC）；透明质酸钠；食品

Determination of Sodium Hyaluronate Content in Food by High Performance Liquid Chromatography

YANG Jing，LIU Chun-li*，LI Jun-wei，HU Jie，LIAO Yu-qiong

（Access（Guangzhou）Testing Technology Service Co.，Ltd.，Guangzhou 510730，China）

Abstract: Purpose：To establish the method for determining the content of sodium hyaluronate in food by reverseliquid chromatography. Methods：For pretreatment，direct extraction or alcohol precipitation extraction could be useddepending on the substrate. An MCI GEL CK08EH column（8mm×300mm，9μm）was used with 1% phosphoricacid as mobile phase. The flow rate was 0.6mL/min，the column temperature was 40℃，and the injection volumewas 20μL. The detection wavelength was 232nm. Results：The correlation coefficient of sodium hyaluronate was1.0000，the spiked recovery rate was 93.2～100.3%， and the relative deviation was 2.71%和3.26%．The detectionlimit was 0.3mg/kg，and the quantification limit was 1.0 mg/kg. Conclusions：This method shows a wide range ofapplicability，good accuracy and precision，and is suitable for the determination of sodium hyaluronate content infood.

Key words: high performance liquid chromatography（HPLC）；sodium hyaluronate；food

中图分类号：TS207.3                   文献标志码：A                          文章编号：1007-7871（2024）06-0036-05

2024，Vol.27，No.6