侯少平，刘春丽，杨 静，黎俊威，胡 杰
（捷通（广州）检测技术服务有限公司，广东 广州 510730）
摘要：目的：建立了一种高效液相色谱-质谱法检测饮料中阿拉伯糖、果糖、葡萄糖、蔗糖、麦芽糖、赤藓糖醇、山梨糖醇和甘露糖醇含量的方法。方法：样品经涡旋混匀，超声提取和稀释后，经高效液相色谱串联质谱分析。流动相为A 相（90 %乙腈+5 %水+5 %异丙醇），B 相（80 %乙腈+20 %水），梯度洗脱，流速为0.85mL/min，柱温为65 ℃，样品进样量为1μL，以保留时间选择离子定性，以外标法定量。结果：阿拉伯糖、果糖、葡萄糖、蔗糖、麦芽糖、赤藓糖醇、山梨糖醇和甘露糖醇在2μg/mL ～100μg/mL 质量浓度范围之间具有良好的线性关系（R2>0.99）；饮料的加标回收率在94.40%～111.94%；方法精密度相对标准偏差RSD%（n=6）在0.68%～4.62%。果汁的加标回收率在90.51%～109.94%；方法精密度相对标准偏差RSD%（n=6）在0.71%～4.82%。结论：本方法可应用于饮料中阿拉伯糖、果糖、葡萄糖、蔗糖、麦芽糖、赤藓糖醇、山梨糖醇和甘露糖醇的测定。
关键词：高效液相色谱-质谱法（HPLC-QDa）；糖和糖醇；饮料
Determination of 5 Sugars and 3 Sugar Alcohols by High Performance Liquid Chromatography-MassSpectrometry Method
HOU Shao-ping，LIU Chun-li，YANG Jing，LI Jun-wei，HU Jie
（Access（Guangzhou）Testing Technology Service Co.，Ltd.，Guangzhou 510730，China）
Abstract: Objective：To develop a high performance liquid chromatography-mass spectrometry（HPLC-QDa）methodfor the determination of arabinose，fructose，glucose，sucrose，maltose，erythritol，sorbitol and mannitol in drink andjuice. Methods：Sample was extracted by vortex oscillated，ultrasonic extracted and diluted with extraction solution，then analyzed by high performance liquid chromatography-mass spectrometry（HPLC-QDa）. The mobile phase A was90% acetonitrile-5% water-5% isopropanol（V/V/V）. The mobile phase B was 80 % acetonitrile -20% water（V/V）.The gradient elution was carried at a flow rate of 0.85mL/min and the column temperature at 65℃，and the sampleinjection volume was 1μL. Sugar and sugar alcohol were qualitatively determined by retention time and selected ions，and were quantified by external standard method. Results：The linear relationship of arabinose，fructose，glucose，sucrose，maltose，erythritol，sorbitol and mannitol was good（R2>0.99）in the concentration range of 2-100μg/mL. Thespiked recoveries of drink are within the range of 94.40%～111.94%. The relative standard deviation（RSD） ofmethod precision was 0.68%～4.62%（n=6）. And the spiked recoveries of juice are within the range of 90.51%～109.94% . The relative standard deviation（RSD） of method precision is 0.71% ～4.82%（n=6）. Conclusion：Thismethod is applicable for the determination of arabinose，fructose，glucose，sucrose，maltose，erythritol，sorbitol andmannitol in drink.
Key words: HPLC-QDa；sugar and sugar alcohol；drink
中图分类号：TS207 文献标志码：Ａ 文章编号：1007-7871（2024）06-0018-08
2024，Vol.27，No.6