苏艳兰

（广东宏科检测技术有限公司，广东 惠州 516000）

摘 要：目的：评定饮料中菌落总数测定结果的不确定度。方法：根据《食品安全国家标准食品微生物学检验菌落 总数测定》（GB4789.2—2022），采用人工加菌污染样品方式建立饮料中菌落总数测定不确定评定方法，对样品稀 释、加样品匀液体积、样品重复性测定等不确定度分量进行分析，评定饮料中菌落总数测定过程中产生的不确定 度。结果：通过分析得出，置信水平为95%时，饮料中菌落总数测定结果的扩展不确定度为0.0328（k=2.20），测定 结果对数值取值区间为2.7029±0.0328，则该样品的菌落总数为 468CFU/mL～544CFU/mL。结论：该评定方法可用 于测定饮料中菌落总数的不确定度评定，也可为食品中菌落总数测定提供依据和参考，提高结果的准确性。

关键词：菌落总数；饮料；不确定度；扩展不确定度；食品

Evaluation of Uncertainty in the Determination of Total Colonies in Beverages

SU Yan-lan

（Guangdong Hongke Testing Technology Co.，Ltd.，Huizhou 516000，China）

Abstract: Objective：Evaluate the uncertainty of the determination results of the total colony count in beverages. Methods：According to GB 4789.2—2022，an uncertainty evaluation method for the determination of total colony count in beverages was established by artificially adding bacteria to contaminate the samples.The uncertainty components such as sample dilution，the volume of the sample homogenate added，and the repeatability of sample determination were analyzed to assess the uncertainty generated during the determination of total colony count in beverages. Results：Through analysis，it was concluded that when the confidence level was 95%，the expanded uncertainty of the total colony count in the beverage was 0.0328（k=2.20）. The logarithmic value range of the measurement result was 2.7029±0.0328. Taking the antilogarithm，the value range was 468 to 544. Therefore，the total colony count of this sample was 468CFU/mL to 544CFU/mL. Conclusion：This method can be used to determine the uncertainty of the total number of bacterial colonies in beverages，and can also provide basis and reference for the total number of bacterial colonies in food，and improve the accuracy of the results.

Key words: total colony；beverage；uncertainty；extended uncertainty；food

中图分类号：TS27        文献标志码：A    文章编号：1007-7871（2025）04-0021-04

2025，Vol.28，No.4