岳虹，赵贞，刘春霞，刘丽君，李翠枝*，邵建波

(内蒙古伊利实业集团股份有限公司，内蒙古呼和浩特010010)

摘要：建立了基于高效液相色谱（HPLC）测定发酵乳中顺丁烯二酸含量的方法。用10%的乙醇溶液做提取剂，甲醇和磷酸盐缓冲液作为流动相，C18 柱进行分离。当顺丁烯二酸的添加含量在1～500mg/kg 时，加标回收率为87.3%～96.7%，相对标准偏差小于4%，定量检出限为0.5mg/kg。该方法具有快速、准确、稳定性好、灵敏度高等优点，可用于发酵乳中顺丁烯二酸含量的检测。关键词：高效液相色谱法；顺丁烯二酸；发酵乳

Determination of Cis-2-Butenedioic Acid in Fermented Milk by HPLC

YUE Hong，ZHAO Zhen，LIU Chun-xia，LIU Li-jun，LI Cui-zhi*，SHAO Jian-bo

（Inner Mongolia Yili Industrial Group Co.，Ltd.，Hohhot 010010，China）

Abstract: A method for the determination of the content of cis-2-Butenedioic acid in fermented milk by high performanceliquid chromatography（HPLC）was developed. Cis-2-Butenedioic acid was extracted by the solution of 10% ethanol. Themethod was performed on C18 column，using methanol and phosphate as mobile phase. Cis-2-Butenedioic acid contentwas in the range of 1～500 mg/kg，the average spiked recoveries were between 87.3% and 96.7% with RSDs（n=6）less than 4%．The limit of quantitation was 0.5mg/kg. The results indicated that this method is accurate，simple andreproducible，and thus suitable for the analysis of the content of cis-2-Butenedioic acid in fermented milk.

Key words: HPLC；cis-2-butenedioic acid；fermented milk

（2015年第18卷第2期）